Fig. 1.

Characterisation of SS-hAFSCs after different incubation timings with or without serum. SS-hAFSCs were incubated for 24 h or 48 h in serum-supplemented or serum-free medium. a, b Membrane markers present on (a) or absent of (b) SS-hAFSCs after incubation in serum (FBS)-supplemented and serum-free (No FBS) conditions. c Cell number after each incubation timing normalised to day 1. n = 3. d Live cells (propidium iodide (PI)-negative) after different incubation timings and conditions. n = 3. *p < 0.05, ns non-significant (p > 0.05), one-way repeated-measures ANOVA with Tukey’s post hoc test