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. 2018 Oct 22;35(2):310–319. doi: 10.3904/kjim.2018.098

Figure 1.

Figure 1.

17β-Estradiol (E2) treatment increases estrogen receptor β (ERβ) expression and induces anti-oxidant enzymes in colonic epithelial cells. E2 in various concentrations (1, 10, 100 nM) and hours (24, 48 hours) did not show difference in estrogen receptor α (ERα) expression measure by Western blot analysis (A) while ERβ increases its expression when 1, 10, or 100 nM of E2 was treated for 48 hours (B). (C) Heme oxygenase-1 (HO-1) has concentration-dependent increment of expression after 48 hours of E2 treatment. (D) NAD(P) H-quinone oxidoreductase-1 (NQO-1) also increases after 48 hours of E2 treatment. (E) Comparison between ERs and antioxidant enzymes, HO-1 and NQO1 expression level treated with 10 nM of E2 for 48 hours. CSS, charcoal-stripped fetal bovine serum; NS, not significant. ap < 0.05 compared with the control (0, white bar) of target gene expression, bp < 0.05 compared with the control (0, black bar) of target gene expression.