Figure 4.

Effects of 3 µM erythromycin on I_hERG tail sensitivity to 30nM BeKm‐1. (a) Representative current traces for effects of 30 nM BeKm‐1 on I_hERG in the absence (ai) or after superfusion of 3 µM erythromycin (aii). Currents were elicited using the protocol shown in the lower panels. (b) Bar charts showing the level of block by 30 nM BeKm‐1 of WT I_hERG tail in the absence (white bar; n = 10) and presence of 3 µM erythromycin (gray bar; n = 6). (c) Bar charts comparing t_halfvalues of inhibition of WT I_hERG tail in the absence (white bar; n = 10) and presence of 3µM erythromycin (gray bar; n = 6). BeKm‐1 effect on I_hERG tail recorded on repolarization to −40mV was calculated as the mean fractional block and plotted against time to derive the value for time to half inhibition (t_half) of I_hERG tail. ** denotes statistically significant difference from WT at p < .01 (unpaired t‐test)