Fig. 1. Down-regulation and modification of Prdx6 in PBMCs of asthmatic patients. (A) PBMCs were prepared from each subject separately and various Prdxs were detected by immunoblotting. Lysates of PBMCs were separated on SDS-PAGE and detected with anti-Prdx6, 1, 2, and 3. β-actin was used as the loading control. Analysis was performed with 6 asthmatic patients and 5 healthy subjects and a single gel is presented as an example. (B) The quantified data from (A) are presented as a graph. The expression level of each Prdx in healthy control was set as 100%. (C) Representative data from 2D-PAGE gel on a non-linear pH (4–7) and Western blotting using anti-Prdx6 for PBMC lysates from 2 healthy subjects and 6 asthmatic patients.

Prdx, peroxiredoxin; PBMC, peripheral blood mononuclear cell; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; 2D-PAGE, 2-dimensional-polyacrylamide gel electrophoresis; IEF, isoelectric focusing; IB, immunoblotting.

^***P < 0.001 vs. healthy controls.