Table 1.
Effects of cyclic D/R on yield, molecular mass and glycosyl composition of cell wall polysaccharides of TR9 and Csol phycobionts. Cell walls were isolated before (T0) and after four D/R cycles, and fractionated sequentially with hot water (HW) and alkali (KOH). Each fraction was submitted to gel filtration chromatography on a Sepharose 4B column. Glycosyl composition analysis of selected fractions was performed by combined GC–MS of the per-O-trimethylsilyl derivatives of the monosaccharide methyl glycosides (Santander et al., 2013)
| Species | Treatment | Fraction | Fraction yield (% of CW mass) | Selected peak | Average molecular mass (kDa) | Monosaccharides (mol %) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ara | Rha | Fuc | Xyl | Man | Gal | Glc | ||||||
| TR9 | T0 | HW | 10.6 | 1 | 75 | 3.4 | 10.1 | – | 4.1 | 3.2 | 55.0 | 24.3 |
| KOH | 35.6 | 3 | 174 | 1.3 | 23.6 | – | 39.5 | 0.6 | 30.7 | 4.4 | ||
| D/R | HW | 14.6 | 2 | 103 | – | 32.5 | – | 8.0 | – | 59.5 | – | |
| KOH | 34.2 | 4 | 19 | 4.3 | 10.9 | – | 49.6 | 3.6 | 29.6 | 1.9 | ||
| Csol | T0 | HW | 13.5 | 5 | 47 | 3.7 | 18.6 | 18.6 | 30.5 | 17.0 | 8.0 | |
| KOH | 18.6 | 6a | 174 | – | – | – | – | – | 4.5 | 95.5 | ||
| 6b | 61 | – | 4.5 | 4.7 | 8.3 | – | 6.4 | 76.1 | ||||
| 6c | 5.2 | 1.2 | 1.5 | 7.3 | 7.8 | 9.1 | 8.1 | 65.2 | ||||
| D/R | HW | 5.5 | – | |||||||||
| KOH | 10.1 | 7a | 103 | – | – | – | – | 20.5 | 15.4 | 64.1 | ||
| 7b | 3.7 | – | – | – | – | – | – | 100 | ||||