Table 2.
Glycosyl linkage analysis of CW polysaccharides of TR9 and Csol microalgae before and after cyclic D/R. Peak fractions of gel permeation chromatography were dialysed, freeze-concentrated, permethylated, depolymerized, reduced and acetylated. The resulting partially methylated alditol acetates were analysed by GC–MS as described by York et al. (1985). Peaks 2 and 5 corresponded to hot-water-soluble polysaccharides and peaks 4, 6 and 7 to KOH-soluble ones (for details see Table 1)
| Linkage | Trebouxia sp. TR9 | Coccomyxa simplex | ||||||
|---|---|---|---|---|---|---|---|---|
| Peak 2 | Peak 4 | Peak 5 | Peak 6a | Peak 6b | Peak 6c | Peak 7a | Peak 7b | |
| (mol %) | ||||||||
| t-Araf | – | 0.8 | – | – | – | – | – | – |
| t-Xylp | 5.7 | 3.0 | 3.5 | 2.1 | 1.1 | – | – | –- |
| 2-Rhap | 10.9 | 1.2 | 7.9 | 3.1 | 0.7 | – | – | – |
| t-Rhap | – | – | 1.3 | 0.3 | – | – | – | – |
| 2-Manp | – | – | 3.7 | 1.5 | 1.1 | – | 0.8 | – |
| 3-Rhap | – | 1.3 | 13.8 | 6.6 | 2.0 | – | – | – |
| t-Manp | – | – | 0.4 | 0.1 | 1.3 | – | – | – |
| 3-Manp | – | – | bql | bql | bql | – | 2.9 | – |
| t-Glcp | 6.1 | 0.5 | – | 4.3 | 7.1 | 16.9 | 48.1 | 91.2 |
| t-Fucp | – | – | 4.0 | – | 0.1 | – | – | – |
| 3-Fucp | – | – | – | 0.3 | 1.9 | – | 3.8 | – |
| t-Galf | – | 10.4 | – | 0.6 | – | – | – | – |
| 4-Fucp | – | – | – | 0.5 | – | – | – | – |
| 3,4-Fucp | – | – | 5.6 | 6.7 | 3.5 | – | – | – |
| t-Galp | – | 1.2 | 2.0 | 1.1 | 4.9 | – | – | – |
| 3-Arap | – | – | 4.3 | 2.7 | – | – | – | – |
| 3-Arap or 4-Araf | – | – | – | – | 1.6 | – | – | – |
| t-Arap | – | – | 0.9 | 0.9 | 0.2 | – | – | – |
| 3-Xylp | – | – | 2.0 | 2.0 | 0.9 | – | – | – |
| 4-Xylp | – | 1.2 | 4.4 | 5.8 | 3.1 | 11.0 | – | – |
| 2-Rhap | – | – | 7.9 | – | – | – | – | – |
| 3,4-Rhap | – | – | 1.5 | 0.4 | – | – | – | – |
| 2,3-Rhap | 14.7 | 1.1 | 6.0 | 4.2 | 0.5 | – | – | – |
| 2-Glcp | – | – | 2.4 | – | – | – | – | – |
| 3-Glcp | – | – | – | 0.3 | 0.4 | – | – | – |
| 3-Glcf | – | – | 2.1 | 1.8 | – | – | – | – |
| 4-Rhap | – | – | – | – | – | – | – | – |
| 4-Manp | – | 5.9 | – | 0.9 | 2.5 | 8.7 | 8.4 | – |
| 3-Galf | 53.2 | – | – | 0.8 | – | – | – | – |
| 3-Hexf* | – | 21.5 | – | – | – | – | – | – |
| 4-Galp | 1.5 | 31.3 | 1.4 | 0.5 | 1.5 | – | 2.0 | – |
| 4-Glcp | 5.3 | 1.4 | 3.2 | 26.0 | 42.0 | 57.4 | 27.1 | 8.8 |
| 6-Galf | 2.7 | – | – | – | – | – | – | – |
| 6-Glcp | – | – | – | – | 0.5 | – | – | – |
| 6-Galp | – | – | 7.1 | 3.2 | 3.8 | – | 6.9 | – |
| 6-Manp | – | – | 0.1 | – | 0.3 | – | – | – |
| 6-Hexf* | – | 2.8 | – | – | – | – | – | – |
| 3-Galp | – | – | 0.8 | – | 3.0 | 5.9 | – | – |
| 2,3-Glcp | – | 2.1 | – | – | – | – | – | – |
| 2,4-Glcp | – | – | – | 0.5 | 1.4 | – | – | – |
| 2,3-Manp | – | – | 1.0 | 1.0 | 0.5 | – | – | – |
| 2,4-Manp | – | – | 9.4 | 4.2 | 2.5 | – | – | – |
| 2,6-Manp | – | 0.3 | 0.1 | – | – | – | – | – |
| 3,4-Manp | – | – | 0.7 | 0.5 | – | – | – | – |
| 3,6-Manp | – | – | – | – | 0.6 | – | – | – |
| 2,3,6-Manp | – | – | 0.1 | 0.1 | bql | – | – | – |
| 2,4,6-Manp | – | – | 0.4 | 0.3 | 0.5 | – | – | – |
| 3,4,6-Manp | – | – | 7.5 | 1.0 | 4.8 | – | – | – |
| 3,4-Galp | – | 1.0 | – | – | – | – | – | – |
| 3,6-Galp | – | 1.4 | 1.6 | 0.2 | 0.6 | – | – | – |
| 3,6-Hexf* | – | 2.2 | – | – | – | – | – | – |
| 4,6-Galp | – | 9.0 | 0.3 | – | – | – | – | – |
| 3,4-Glcp | – | – | – | 5.0 | 4.0 | – | – | – |
| 4,6-Glcp | – | – | 0.3 | 5.1 | 4.0 | – | – | – |
| 2,3,4-Glcp | – | – | – | 0.6 | 06 | – | – | – |
| 3,4,6-Galp | – | 0.5 | – | – | – | – | – | – |
| 3,4,6-Glcp | – | – | – | 1.0 | 0.6 | – | – | – |
*Probably galactofuranosyl residues; – not detected; bql, below the quantification level.