Figure 3.
Effects of lung cancer condition and ATTM treatment on m6A related protein expression. (A) Confirmed LUAD patient tumor tissues and normal adjacent tissues, as well as (B) A549 cells treated with 60 μM ATTM for 24 h and normal cells, were collected and split. Total proteins were used for Western blot assay to measure the expressions of METTL3, METTL14, and FTO. Corresponding quantifications were shown as (C,D), respectively. Data are presented as mean ± SD. n = 2. *P < 0.05, **P < 0.01 vs. Control group. (E) A549 cells transfected with shMETTL3 or shGFP were collected and gene expression was profiled by high throughput sequencing. Representative genes of eukaryotic translation initiation factors (eIFs) were analyzed and shown as the maximum vs. minimum of the ratio of shMETTL3 to shGFP (GSE76367).