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The impact of primers on base editing quantification using BEAT. The A-to-G editing frequencies at (A) A5 and (B) A7 of site 1 were measured and quantified using two primer pairs (Site1-F1/R1 and Site1-F2/R2). The A-to-G editing frequencies at (C) A5 and (D) A7 of site 5 were measured and quantified using two primer pairs (Site5-F1/R1 and Site5-F2/R2). NS, not significant.
