Table 2.

List of PCR Primers for the Ano5 Plasmids and gRNA Target Sites

Ano5 plasmids	F: 5′ GCGATTCAATTTGTTCCTGAG
	R: 5′ CCTGAATGCAAACTGTGTCAA
Site 1	F1: 5′ AACCAGTGTCAGGGAGCTGT
	R1: 5′ ATCCACAGCAACACCCTCTC
	F2: 5′ AGGACGTCTGCCCAATATGT
	R2: 5′ CAGCCCCATCTGTCAAACTG
Site 2	F: 5′ CAGGAATATCTGTGTGTGAGCCATA
	R: 5′ AGGAGTTCGCAGTGAGCCG
Site 5	F1: 5′ AAGGTTTTGGGCTTCATTCC
	R1: 5′ CGCCTGGTCACATTGACTTT
	F2: 5′ CTCAAAACGGTAGAGCAGGC
	R2: 5′ AGGCTGGTCTTGAACTCCTG