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. Author manuscript; available in PMC: 2020 Mar 9.
Published in final edited form as: J Am Chem Soc. 2019 Apr 1;141(14):5788–5797. doi: 10.1021/jacs.8b13157

Figure 7.

Figure 7.

Characterization of SAM-adducts. A). MRM scan of SAH (transition of 358 to 136) in the NosN assay on compound 7 (red trace) and compound 8 (blue trace). The peaks at 1.2 min correspond to the SAH produced by methylation. The peaks at 3.5 and 4.1 min correspond to products that can be fragmented to SAH; B). MS analysis of SAM-adduct I; C). MS analysis of SAM-adduct II. Each reaction contained 1 mM compound 7 or 8, 2 mM Ti(III) citrate, 50 μM NosN, and was initiated with 1 mM SAM and incubated for 1h.