Table 5.
Microbial diversity and changes in the periodontal pockets (PP) and root canals (RC) of combined endodontic-periodontal lesions before (B) and after (A) chemomechanical preparation in 15 subjects
| MICROORGANISM/GENUS PROBE | PPB | PPA | PPA/PPB | RCB | RCA | RCA/RCB |
|---|---|---|---|---|---|---|
| Actinomyces israelii* | 0.09 | 0.04 | 0.50 | 0.01 | 0.00 | 0.50 |
| Actinomyces israelii** | 13.30 | 13.30 | 1.00 | 0.00 | 0.00 | 1.00 |
| Atopobium sp oral taxon 199* | 0.00 | 0.00 | 1.00 | 1.26 | 0.02 | 0.02 |
| Bacteroidaceae [G-1] sp. oral taxon 272* | 0.06 | 0.04 | 0.71 | 5.13 | 0.16 | 0.03 |
| Desulfobulbus sp oral taxon 041*** | 18.30 | 22.85 | 1.25 | 1.30 | 0.84 | 0.65 |
| Dialister invisus* | 0.08 | 0.10 | 1.22 | 0.68 | 0.37 | 0.55 |
| Dialister pneumosintes* | 0.02 | 0.24 | 8.33 | 0.29 | 0.06 | 0.23 |
| Enterococcus faecalis* | 6.81 | 19.88 | 2.92 | 22.02 | 46.08 | 2.09 |
| Enterococcus faecalis** | 0.00 | 0.00 | 1.00 | 20.00 | 0.00 | 0.00 |
| Erysipelothrichaceae [G-1]sp oral taxon 905*** | 0.00 | 0.00 | 1.00 | 1.92 | 0.00 | 0.01 |
| Eubacterium brachy* | 1.96 | 1.38 | 0.71 | 0.98 | 0.68 | 0.70 |
| Eubacterium nodatum* | 0.14 | 0.18 | 1.27 | 0.10 | 0.06 | 0.64 |
| Eubacterium saphenum* | 1.23 | 1.29 | 1.05 | 0.43 | 0.56 | 1.30 |
| Filifactor alocis* | 1.86 | 2.32 | 1.25 | 2.50 | 0.36 | 0.15 |
| Fretibacerium fastidiosum* | 0.43 | 0.45 | 1.05 | 2.40 | 0.60 | 0.25 |
| Fusobacterium nucl. ssp nucleatum* | 0.29 | 0.17 | 0.60 | 0.01 | 0.00 | 0.50 |
| Fusobacterium nucl. ssp nucleatum** | 53.30 | 0.00 | 0.00 | 53.80 | 73.30 | 1.36 |
| Mogibacterium timidum* | 0.85 | 0.83 | 0.98 | 2.78 | 0.17 | 0.06 |
| Parvimonas micra* | 9.00 | 9.42 | 1.05 | 14.56 | 1.65 | 0.11 |
| Parvimonas micra** | 0.00 | 40.00 | 4001 | 33.30 | 0.00 | 0.00 |
| Parvimonas sp. oral taxon 110* | 2.16 | 0.30 | 0.14 | 0.32 | 0.61 | 1.88 |
| Peptostr.[11][G-1] sp oral taxon 383*** | 0.06 | 0.41 | 6.00 | 2.74 | 0.08 | 0.03 |
| Peptostr. [13][G-1] sp oral taxon 113* | 1.17 | 1.83 | 1.56 | 3.13 | 0.20 | 0.07 |
| Peptostreptococcus stomatis* | 0.97 | 1.05 | 1.08 | 2.66 | 0.30 | 0.12 |
| Porphyromonas endodontalis* | 0.66 | 0.01 | 0.03 | 0.09 | 0.01 | 0.20 |
| Porphyromonas gingivalis* | 0.12 | 0.02 | 0.23 | 0.11 | 0.77 | 6.50 |
| Porphyromonas gingivalis** | 40.00 | 6.70 | 0.17 | 0.00 | 0.00 | 1.00 |
| Prevotella intermedia* | 0.00 | 0.00 | 1.00 | 0.00 | 0.00 | 1.00 |
| Prevotella intermedia** | 40.00 | 33.30 | 0.83 | 66.70 | 6.70 | 0.10 |
| Prevotella nigrescens* | 0.01 | 0.00 | 0.50 | 0.02 | 0.92 | 31.00 |
| Pseudoramibacter alactolyticus* | 0.36 | 1.00 | 2.73 | 1.09 | 0.12 | 0.12 |
| Rothia mucilaginosa* | 0.95 | 0.61 | 0.65 | 0.16 | 0.61 | 3.65 |
| Stomatobaculum sp oral taxon 373*** | 0.26 | 0.29 | 1.11 | 3.67 | 0.29 | 0.08 |
| Streptococcus constellatus* | 2.54 | 1.93 | 0.76 | 1.27 | 0.32 | 0.26 |
| Streptococcus constellatus** | 40.00 | 20.00 | 0.50 | 13.30 | 0.00 | 0.00 |
| Streptococcus salivarius/vestibularis* | 0.27 | 0.38 | 1.39 | 0.53 | 2.24 | 4.17 |
| Streptococcus salivarius/vestibularis** | 0.00 | 0.00 | 1.00 | 13.30 | 6.70 | 0.50 |
| Tannerella forstythia* | 1.78 | 0.23 | 0.13 | 0.34 | 0.07 | 0.23 |
| Treponema denticola* | 0.06 | 0.00 | 0.14 | 0.01 | 0.14 | 7.50 |
| Treponema maltophilum* | 0.05 | 0.01 | 0.33 | 0.02 | 0.01 | 0.67 |
| Treponema socranskii * | 0.02 | 0.01 | 0.67 | 0.01 | 0.05 | 3.00 |
| Actinomyces Genus probe* | 0.75 | 1.72 | 2.28 | 1.95 | 4.06 | 2.08 |
| Desulfobulbus Genus probe* | 1.28 | 1.27 | 0.99 | 0.06 | 0.03 | 0.57 |
| Dialister Genus probe 1* | 0.00 | 0.01 | 2.00 | 0.01 | 0.00 | 0.50 |
| Dialister Genus probe 2* | 0.00 | 0.00 | 1.00 | 0.02 | 0.01 | 0.67 |
| Enterococccus Genus probe* | 0.12 | 0.29 | 2.31 | 0.24 | 0.87 | 3.52 |
| Erysipelothrichaceae Genus probe* | 0.00 | 0.00 | 1.00 | 0.01 | 0.00 | 0.50 |
| Eubacterium Genus probe 1* | 0.49 | 0.34 | 0.70 | 0.18 | 0.39 | 2.11 |
| Eubacterium Genus probe 2* | 0.02 | 0.04 | 1.67 | 0.03 | 0.01 | 0.50 |
| Filifactor Genus probe* | 0.13 | 0.18 | 1.36 | 0.21 | 0.03 | 0.18 |
| Fretibacerium Genus probe* | 0.09 | 0.14 | 1.50 | 0.23 | 0.09 | 0.42 |
| Fusobacterium Genus probe* | 4.36 | 2.64 | 0.61 | 0.17 | 2.12 | 11.83 |
| Mogibacterium Genus probe* | 0.04 | 0.03 | 0.80 | 0.10 | 0.00 | 0.09 |
| Parvimonas Genus probe* | 1.83 | 0.23 | 0.13 | 2.66 | 0.23 | 0.09 |
| Peptostreptococcus Genus probe* | 0.04 | 0.04 | 1.00 | 0.10 | 0.02 | 0.27 |
| Porphyromonas Genus probe* | 0.02 | 0.00 | 0.33 | 0.01 | 0.00 | 0.50 |
| Prevotella Genus probe* | 0.01 | 0.00 | 0.50 | 0.01 | 0.00 | 0.50 |
| Rothia Genus probe* | 0.05 | 0.80 | 13.50 | 0.02 | 0.06 | 2.33 |
| Staphylococcus Genus probe* | 0.93 | 1.13 | 1.21 | 2.88 | 10.31 | 3.57 |
| Streptococcus Genus probe* | 10.84 | 3.26 | 0.30 | 1.95 | 3.94 | 2.02 |
| Tannerella Genus probe* | 0.04 | 0.00 | 0.20 | 0.01 | 0.00 | 0.50 |
| Treponema Genus probe 3* | 0.00 | 0.00 | 1.00 | 0.00 | 0.06 | 7.00 |
| Treponema Genus probe 4* | 0.05 | 0.02 | 0.50 | 0.03 | 0.01 | 0.50 |
| Treponema Genus probe 5* | 0.04 | 0.01 | 0.40 | 0.01 | 0.06 | 3.50 |
*
NGS
**
culture
***
phylotype
Peptostrep = Peptostreptococcaceae