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. Author manuscript; available in PMC: 2020 Mar 9.
Published in final edited form as: Prostate. 2017 Mar 1;77(7):749–764. doi: 10.1002/pros.23311

Fig. 3.

Fig. 3.

Flow cytometry analysis of PSMA expression on live cells. Specificity and labeling intensity of the mAbs 5D3 (A) and 5B1 (B) were compared to J591 (C) using LNCaP (PSMA-positive) and PC-3 (PSMA-negative) cell lines of prostate origin. Harvested cells were incubated with 5 μg/ml of each mAb and binding detected by a secondary antibody conjugated to Alexa Fluor 647 using an LSRII flow cytometer. A minimum of 30,000 cells were analyzed for each sample using FlowJo software. While staining on PC-3 cells was negative, staining profiles of LNCaP cells suggest comparable performance for all three mAbs tested. According to the indicated median fluorescence intensity, 5D3 revealed the strongest binding affinity toward PSMA on LNCaP cells.