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. 2020 Mar 4;13:1921–1929. doi: 10.2147/OTT.S223542

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© 2020 Yuan et al.

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Hsa_circ_0068033 acts as a sponge of miR-659 to regulate the progression of BC. (A) Distribution of hsa_circ_0068033 in the nuclear and cytoplasmic fractions of MCF-7 and MDA-MB-231 cells analyzed through qRT-PCR. (B) The expression of hsa_circ_0068033 was upregulated in BC tissues compared with non-cancerous tissues. (C) MiR-659 levels in the two cell lines transfected with PLCDH-cirR0068033 and the miR-659 mimic. (D) Luciferase assay of MDA-MB-231 cells co-transfected with the miR-659 mimic and luciferase reporter containing hsa_circ_0068033-wild sequences (cirRNA-wt) or -mutant sequences (cirRNA-mut). (E) CCK-8 analysis. (F) Wound healing migration assay. (G) Transwell assay. (H) TUNEL apoptosis assay to verify the effects of hsa_circ_0068033 as a sponge of miR-659. *P<0.05, **P<0.01.