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. Author manuscript; available in PMC: 2020 Mar 9.
Published in final edited form as: Nature. 2019 Dec 18;577(7788):121–126. doi: 10.1038/s41586-019-1842-7

Extended Data Fig. 7 |. Loss of PAF1 has minimal effect on the functionality of cancer-associated ENL mutants.

Extended Data Fig. 7 |

a, Western blot analysis of co-immunoprecipitation using the M2 anti-Flag antibody in lysates from HEK293T cells expressing the indicated Flag–ENL constructs. Results are representative of three independent experiments. b, c, mRNA expression (b) and western blot (c) analysis showing the knockdown efficiency of short interfering (si)RNAs that target PAF1 in HEK293 cells. Independent experiments were repeated twice with similar results. d, e, ChIP-qPCR analysis of Flag–ENL at selected ENL target genes in control (siCtrl) or PAF1 knockdown (siPAFl) HEK293 cells. f, mRNA expression analysis (normalized to GAPDH) of selected ENL target genes in control (siCtrl) or PAF1 knockdown (siPAFl) HEK293 cells. Data in d-f represent means ± s.e.m.; n = 3 technical replicates. For gel source data in panels a, c, see Supplementary Fig. 1.