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. Author manuscript; available in PMC: 2020 Mar 9.
Published in final edited form as: Nature. 2019 Dec 18;577(7788):121–126. doi: 10.1038/s41586-019-1842-7

Fig. 3 |. Acylation-reading activity is required for enhanced chromatin occupancy by ENL T mutants.

Fig. 3 |

a, Structure (Protein DataBank code 5J9S) of the ENL YEATS domain (blue ribbon) bound to a histone H3 peptide comprising an acetylated lysine 27 residue (H3K27ac, yellow), showing a key ENL residue (Y78, green) that mediates recognition of histone acetylation and the region that is mutated in cancer (red). b, Genome browser view of the ChIP–seq signals from different Flag–ENL proteins at the genes indicated at the bottom in HEK293 cells. c, Box plots showing the fold change (FC) in Flag–ENL ChIP–seq signals (relative to wild-type ENL) at peak regions that bear enhanced occupancy of ENL T mutants (n = 54) in HEK293 cells. Centre lines represent medians, the box limits are the 25th and 75th percentiles and the whiskers show the range of values. P-values were obtained using paired two-tailed t-tests. d, mRNA expression analysis (normalized to GAPDH) of selected genes in HEK293 cells expressing the indicated constructs. Data represent means from n = 2 technical replicates, and results are representative of three independent experiments.