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. 2020 Mar 4;13:1909–1919. doi: 10.2147/OTT.S241632

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© 2020 Wang et al.

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Berberine (BBR)-mediated reversal of doxorubicin (ADR) resistance is associated with inhibition of autophagy. (A) ADR-resistant MCF-7 (MCF-7/ADR) cells were treated with BBR and/or ADR. Combined ADR and BBR treatment significantly decreased the number of autophagosomes in the MCF-7/ADR cells (BBR, 100 µM; ADR, 0.517 µM). Yellow arrows indicate Autophagosome. (B) MCF-7/ADR cells were treated with BBR and/or ADR. Western blotting analysis showed that combined ADR and BBR treatment led to a significant decrease in the expression of LC3I/II and an increase in that of p62 in MCF-7/ADR cells (BBR, 100 µM; ADR, 0.517 µM). (C) After treatment with rapamycin, MCF-7/ADR cells were then treated with BBR and/or ADR. In the presence of rapamycin, BBR treatment induced no significant change in the activity of MCF-7/ADR cells (BBR, 100 µM; ADR, 0.517 µM; rapamycin, 50 nM). N/A, not significant; ***p<0.001 (Student’s t-test).