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Viability of A549 cells after silencing VEGF using free siRNA or the polyplex for 4 h followed by treatment with 5-FU for 48 h. A final concentration of 100 nM siRNA and 400 µM of 5-FU were used in A549 cells and shown as mean ± SD (n = 3). ***p < 0.05 compared with the control.
