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. 2020 Feb 17;11:354–369. doi: 10.3762/bjnano.11.26

Figure 2.

Figure 2

(A) FTIR spectra of free siRNA, PVI and the polyplex recorded in ATR mode between 4000 and 400 cm−1. (B) Heat flow profiles of pristine PVI and polyplex in nitrogen atmosphere at a scan rate of 5 °C·min−1. (C) Heparin displacement assay for the polyplex at different concentrations of heparin. Lane 1: free siRNA; lane 2: 60 ng/mL heparin only; lane 3: heparin/polyplex ratio (w/w) of 0.5; lane 4: heparin/polyplex ratio (w/w) of 1, lane 5: heparin/polyplex ratio (w/w) of 1.5; lane 6: heparin/polyplex ratio (w/w) of 2; lane 7: heparin/polyplex ratio (w/w) of 2.5; lane 8: heparin/polyplex ratio (w/w) of 3. The absence of free siRNA in the polyplex lanes treated with heparin shows the stability of the complex formed.