Figure 4.

The endosomal escape of the polyplex after 4 h of incubation in A549 cells visualized using endotracker (stains early endosomes green) and the red fluorescence from Cy-3 labeled siRNA observed with confocal laser scanning microscopy. The co-localization of the Cy3-labeled siRNA and endosome is observed as yellow fluorescence (red arrows). The green emission was recorded at 488 nm while the red emission was captured at 568 nm. The blue fluorescence of Hoechst 33258 was recorded at 405 nm.