Fig. 1.

(A) CAD ​design of cartilage-on-a-chip device featuring six individually addressable circular chambers on one object slide, enabling multiplexing of experiments. (B) Photograph of an actual cartilage-on-a-chip device with glass slide as the top layer and PDMS slab with microstructures as the bottom layer showing loading of cell-laden hydrogel in the top chamber using a pipette tip. (C) Lift side showing overview picture of three cell culture chambers and right side showing one individual culture chamber featuring CMFDA-stained primary equine chondrocytes cultivated on-chip for 21 days. Scale bars 5 ​mm and 500 ​μm. (D) Intact cell-laden fibrin hydrogel clot released from the device before downstream analyses and (E) histological section of chondrocytes-on-a-chip. Scale bar 100 ​μm. CMFDA, 5-chloromethylfluorescein diacetate