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Comparison of diffusion distance and speed of (A) 40-kDa-sized and (B) 0.3-kDa-sized biomolecules into the fibrin hydrogel at various time points up to 24 h. A nutrient gradient is formed for the 40-kDa-sized molecule, stabilizing in the time frame of 12 h–24 h, while the 0.3-kDa-sized molecule diffuses rapidly into the culture chamber and levels out at 75% of feed concentration.
