Figure 4.

TS silencing inhibits the proliferation of TNBC cells. A. Immunoblot analysis of TS expression in MCF-10A, MCF-7, MDA-MB-231, BT-549 and Hs578T cells. B. TS silencing in the indicated cells. C. TS silencing reduces the viability of TNBC cells. D-G. Flow cytometry analysis of the effect of TS silencing on the proliferation and apoptosis of breast cancer cells. H. Immunoblot analysis of the relative expression levels of cell cycle regulators. I. Female NOD/scid mice were implanted with MDA-MB-231/shCon and MDA-MB-231/shTS cells, respectively. At the end of the experiment, their tumors were dissected and photoimaged. J. The tumor weights were measured. Data are representative images, or expressed as the mean ± SEM of each group of cells from three separate experiments. *P<0.05, **P<0.01, ***P<0.001 vs. the control.