Figure 7.

MyD88 overexpressed SCC4 cells grew faster and were more resistant to cetuximab in vivo. (A) SCC4 cells transfected with overexpression vectors of MyD88 or control vectors were injected into nude mice. Cetuximab was peritoneally injected 7 days later and tumor volume was measured every 3 days. The relative mRNA expression of iNOS (B), COX2 (C) and TNF-α (D) extracted from tumor tissues was measured. (E) The expression of TLR4, MyD88 and TRIF in xenograft was measured by RT-PCR. SCC4 cells transfected with overexpression vectors of MyD88 (F) or control vectors (E) were injected into nude mice. Cetuximab and TAK242 were peritoneally injected 7 days later and tumor volume was measured every 3 days. Data were represented as mean ± SD of at least three independent experiments; *P<0.05, **P<0.01; ***P<0.001.