Serum concentration of C3 and C4 and expression of IL-1β, IL-6, and TNF-α in serum of LN patients. A. The concentration of C3 and C4 was lower in serum from active LN patients compared with that in serum from inactive LN patients. B. ELISA showed that serum expression of IL-1β, IL-6, and TNF-α was markedly upregulated in active LN patients compared to inactive LN patients. Data are mean ± SD from triplicate experiments, *P<0.05. C3: complement component 3, C4: complement component 4, IL-1β: interleukin 1 beta, IL-6: interleukin 10, TNF-α: tumor necrosis factor α, ELISA: enzyme-linked immunosorbent assay.