Skip to main content
. 2020 Feb 1;13(2):324–331.

Figure 4.

Figure 4

The effect of miR-203 overexpression on the TRAF6-induced IL-β, IL-6, TNF-α activation of HRMCs and HK-2 cells. A. qRT-PCR results revealed that miR-203 was efficiently overexpressed by transfection of miR-203 mimic in HRMCs and HK-2 cells, with U6 as an internal control. B. TRAF6 treatment significantly elevated the concentration of IL-1β, IL-6, and TNF-α compared to the control group. C. HRMC cells transfected with miR-203 mimics showed lower IL-1β, IL-6, and TNF-α expression than that in the mimic control group. D. Transfection of HK-2 cells with miR-203 mimics resulted in significantly decreased expression of IL-1β, IL-6, and TNF-α compared to the control group. Data are mean ± SD from triplicate experiments, *P<0.05. TRAF6: TNF receptor associated factor 6, HRMCs: human renal mesangial cell line, HK-2: human renal tubular epithelial cell line.