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. 2020 Feb 1;13(2):230–238.

Figure 1.

Figure 1

Workflow and quantitative performances of the ddPCR panels for IDH1 and TERT promoter mutation analysis. A. First, DNA templates and primer pairs were mixed with ddPCR Supermix. Then, generating droplets using Automated Droplet Generator. Next, the PCR mixture for each assay was compartmentalized into about 20000 droplets for independent PCR reactions. Finally, droplets were scanned and analyzed using QX200 Droplet Reader one by one. B. The test in reference-standard plasmid with serial variant IDH1 mutants (R132H, R132S, R132G, R132C, R132L) and TERT promoter mutants (C228T and C250T) using IT-ddPCR.