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TM suppresses the CSC characteristics of HNSCC cells. A. Representative image of tumorsphere formation in HN4 and CAL27 cells treated with or without TM (2 µg/ml) for 7 days. Scale bar =100 μm. B. Measurement of tumorsphere formation (mean ± SD from 3 separate experiments) in CAL27 and HN4 cells, *P<0.05. C. Western blot analysis of CD44, Bmi-1 and Oct-4 protein levels of HN4 and CAL27 cells with or without of TM (2 µg/ml) treatment for 48 hours.
