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. 2020 Feb 15;12(2):478–492.

Table 1.

Sequences of RT-qPCR primers

Primers Sequences Length (bp)
α-SMA (human) F: 5’-GACGAAGCACAGAGCAA-3’ 150
R: 5’-GTGGGATGCTCTTCAG-3’
CD31 (human) F: 5’-CACTGAAGACGTCGAAT-3’ 150
R: 5’-CCAGACTCCACCACCTTACT-3’
VEGFA (human) F: 5’-GTACCCTGATGAGATCGAGTA-3’ 151
R: 5’-TGAGGTTTGATCCGCATA-3’
TGF-β (human) F: 5’-CAGAGTGGTTATCTTTTGA-3’ 150
R: 5’-TAGTGAACCCGTTGATGT-3’
PDGF (human) F: 5’-GCACCAACGCCAACTTCCT-3’ 171
R: 5’-ACCGTGGCCTTCTTAAAGATTG-3’
GAPDH (human) F: 5’-GCACCGTCAAGGCTGAGAAC-3’ 137
R: 5’-TGGTGAAGACGCCAGTGGA-3’
c-Myc (Rat) F: 5’-ACCCCCCTCCACAAGGAA-3’ 150
R: 5’-ACGTTGTGTGTCCGCCTCTT-3’
Bcl-2 (Rat) F: 5’-CGGTGGTGGAGGAACTCTTC-3’ 165
R: 5’-TGTGCAGATGCCGGTTCA-3’
Bad (Rat) F: 5’-TGAGGAAGATGAAGGGATGGA-3’ 150
R: 5’-CGAGGAAGTCCCTTGAAGGAA-3’
p53 (Rat) F: 5’-AGAGGAAGAAAATTTCCGCAAA-3’ 150
R: 5’-CCACGGATCTTAAGGGTGAAATAT-3’
GAPDH (Rat) F: 5’-GGCACAGTCAAGGCTGAGAATG-3’ 142
R: 5’-ATGGTGGTGAAGACGCCAGTA-3’