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. 2020 Feb 15;12(2):343–360.

Figure 3.

Figure 3

The impact of Icariin on TGF-β1-induced EMT markers expression by western blot and quantitative real-time PCR. 16HBE cells were pretreated with or without Icariin (low, medium and high dose) for 6 h and then treated with or without 10 ng/ml TGF-β1 for 24 h. A. Subjected to western blot analysis of E-cadherin, α-SMA and N-cadherin expression. Data were expressed as fold change in protein expression normalized to β-actin expression. B. The expressions of E-cadherin, α-SMA and N-cadherin mRNA were determined by quantitative real-time PCR, data were expressed as fold change in mRNA expression normalized to GAPDH, with respect to the control group. #P<0.05, ##P<0.01 versus control group, *P<0.05, **P<0.01 versus TGF-β1 group.