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. 2020 Mar 2;11:148. doi: 10.3389/fpls.2020.00148

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Copyright © 2020 Cifrová, Oulehlová, Kollárová, Martinek, Rosero, Žárský, Schwarzerová and Cvrčková

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Pavement cell shape in the adaxial epidermis of 5 days after germination (DAG) cotyledons of actin nucleator mutants and wt plants. (A) Representative images of wt, fh1-1, arpc5-1, and fh1-1 arpc5-1 cotyledon epidermis. Epidermal continuity defects are marked by arrowheads. (B) Selected morphometric parameters of cotyledon pavement cells as determined by the semi-manual approach (see Methods): cell circularity and solidity as measures of cell shape complexity, cell area, aspect ratio as a measure of shape anisotropy. (C) Average cell lobe number and equatorial lobe width as determined by PaCeQuant. (D) Scatter plot of PCA results for five morphometric parameters (cell circularity, solidity and area, lobe number, and average equatorial lobe width) determined by PaCeQuant, shown at an arbitrary scale. Values labeled by the same letters in (B, C) do not differ significantly (p < 0.05).