Figure 5.
Pavement cell shape in the adaxial epidermis of 1st true leaves of 14 days after germination (DAG) actin nucleator mutants and wt plants. (A) Representative images of wt, fh1-1, arpc5-1, and fh1-1 arpc5-1 true leaf pavement cells. (B) Selected morphometric parameters of leaf epidermal pavement cells as determined by the semi-manual approach (see Methods): cell circularity and solidity as measures of cell shape complexity, cell area, aspect ratio as a measure of shape anisotropy. (C) Average cell lobe number and equatorial lobe width as determined by PaCeQuant. (D) Scatter plot of PCA results for five morphometric parameters (cell circularity, solidity and area, lobe number, and average equatorial lobe width) determined by PaCeQuant, shown at an arbitrary scale. Values labeled by the same letters in (B, C) do not differ significantly (p < 0.05).