Figure 1.

Overview of single-cell TIPseq workflows. The single-cell TIPseq procedure consists of five steps: cell sorting, WGA, a quality control check, vectorette PCR for L1Hs insertion site amplification and next-generation sequencing. Pink, MDA WGA with or without L1 primer (MDA); light blue, MALBAC WGA with or without L1 primer (MALBAC); red, MDA WGA followed by restriction enzyme digestion and ligation with vectorette adaptors (MDA-D); orange, MDA WGA followed by end repair, dA tailing and ligation with dT vectorette adaptor (MDA-T); dark blue, MALBAC WGA followed by ligation with dT vectorette adaptor (MALBAC-T). Circles represent WGA using random hexamers only (R); squares represent WGA using random hexamers and the L1 primer (RL).