Figure 4.

Total number of insertions predicted. (a) Including all predictions. (b) Including only known L1Hs insertion sites including the reference L1Hs and published polymorphic L1Hs. Black dashed line, bulk DNA regular TIPseq; red, MDA WGA followed by restriction enzyme digestion and ligation with vectorette adaptors (MDA-D); orange, MDA WGA followed by end repair, dA tailing and ligation with dT vectorette adaptor (MDA-T); dark blue, MALBAC WGA followed by ligation with dT vectorette adaptor (MALBAC-T). Circles, WGA using random hexamers only (R); squares, WGA using random hexamers and L1 primer (RL). Arrows indicate the MDA sample included in the vectorette PCR (both MDA-D and MDA-T) and next-generation sequencing stages that had less than perfect QC (corresponds to the same sample indicated in figure 2).