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. 2020 Feb 10;375(1795):20190417. doi: 10.1098/rstb.2019.0417

Figure 1.

Figure 1.

Pol IV-dependent 21–22 nt siRNAs (a) Per cent of TE siRNAs in different size classes in wild-type Columbia (wt Col) seedlings, inflorescence and pollen tissue. Rep A and Rep B are distinct biological replicates, 1n is normal haploid pollen and 2n is diploid pollen derived from a 4n parent [15]. (b) Reads per million (RPM) counts of TE siRNAs in wt Col and pol IV mutant plants. (c) 24 nt siRNA accumulation in inflorescence from all identified 24 nt siRNA-producing clusters (left) or clusters specifically identified as dependent on Pol IV (right). Above zero are sense siRNAs, and below are antisense. (d) 21–22 nt siRNA accumulation in inflorescence from all identified 21–22 nt siRNA-producing clusters (left) or clusters identified that are dependent on Pol IV (right). (e) Overlap between the regions of the genome where Pol IV produces 24 nt versus 21–22 nt siRNAs.