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. 2020 Feb 10;375(1795):20190417. doi: 10.1098/rstb.2019.0417

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© 2020 The Author(s)

Published by the Royal Society. All rights reserved.

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Figure 3. — Pathway for Pol IV-dependent siRNA production. (a,b,d,e) Heatmap of read counts of 15–34 nt small RNAs in inflorescence from Pol IV-dependent clusters in various mutant combinations. 24 nt siRNAs clusters are used in (a,d) and 21–22 nt siRNA clusters are used in (b,e). (a,b) uses DCL family mutants. dcl2/3/4 refers to the dcl2/dcl3/dcl4 triple mutant. (d,e) uses RDR family mutants, and rdr1/2/6 refers to the rdr1/rdr2/rdr6 triple mutant. Insets of (d,e) show accumulation of 24 nt and 21–22 nt siRNAs, respectively, with abundance averaged across replicates. (c) Developmental expression pattern of DCL3 protein according to ref. [23] and analysed in the eFP-RNA-Seq Browser [24].