Fig. (1).

Effect of triptolide on testis and sperm morphology and physiological sperm parameters. The mice received either physiological saline solution (control) or administered triptolide with i.g. of 50 μg/kg Body weight/day. Mice were euthanized by cervical dislocation at 35 days after treatment. Testes and spermatozoa from the cauda epididymis were harvested quickly. After testis sections were stained with hematoxylin and eosin (H&E), testis morphology (A) (B) was observed under optical microscope. Body (C) and testicular weights (D) were measured using an electronic balance. Sperm morphology (E) (F) was also observed under optical microscope. Sperm abnormality rate (G) Sperm motility (H), and sperm density (I) were analyzed using a CASA system. At least 200 spermatozoa were counted for each assay. Data are presented as the mean ± SE, (n = 6). **P < 0.01 compared with control.