Table 1. qPCR primers of examined genes.
Gene ID | Tm(°C) | Length (bp) | Primers (5ʹ→3ʹ) |
---|---|---|---|
ENSMUSG00000099722(lnc1) | 60 | 192 | F: TCCAGCTTCACCTTGTCACG R: ACGTCTGTTCCGTCATTCCA |
ENSMUSG00000106652(lnc2) | 60 | 93 | F: GCACAGTGAGAGGAGCATTG R: GATCGGCACCTGATTGGTCT |
ENSMUSG00000105299(lnc3) | 60 | 222 | F: GGCAACCAGAATCTAGCACT R: GCTGTACATTTTCCTCGGGC |
ENSMUSG00000099869(lnc4) | 60 | 118 | F: GGAGGGCTGGGATTCACAC R: TCCTCCTCATGCACTTTCACA |
ENSMUSG00000021130(lnc5) | 60 | 134 | F: GGGTTTAGTGGGGTCAGTCC R: AGGGTGGAAAAGAACGTCCA |
ENSMUSG00000092341(lnc6) | 60 | 103 | F: TTCAAGGGGCCAGAGAATCC R: CTTCCCAATCCCCACACTGAA |
mmu_circ_0000335(cicr1) | 60 | 146 | F: GAAGCGACCCAGTCAATCCT R: CATGTGAGCCTCCTCTACGC |
mmu_circ_0000947(cicr2) | 60 | 161 | F: GTGGTGTTTGCTATGCTGGC R: GAGAAGACCGACCCGTTTGT |
Prm1 | 60 | 207 | F: GCTCACAGGTTGGCTGGCTCG R: TGGTGTATGAGCGGCGGCGA |
Prm2 | 60 | 337 | F: GAGCGCGTAGAGGACTATGG R: CAGACATCGACATGGAATGG |
Tnp1 | 60 | 81 | F: ACCAGCCGCAAGCTAAAGAC R: GCTTCCACCTCTCTTGACGC |
Tnp2 | 60 | 253 | F: CACACCAGTAACCAGTGCAAT R: TAGCTCTGTGAGTCCGTTTCC |
β -actin | 60 | 154 | F: GGCTGTATTCCCCTCCATCG R: CCAGTTGGTAACAATGCCATGT |