Table 1.
Primers used for amplification and sequencing of the mitochondrial genome of Asotana magnifica.
| fragment | gene or region | primer name | sequence (5′-3′) | length (bp) |
|---|---|---|---|---|
| F1 | ATP6 | SSF1 | GTCAACNTTWAGTAGTCYTC | 787 |
| SSR1 | GCTACTDCTGTTTCTAGG | |||
| F2 | ATP6-12S | SSF2 | TCTCATTCAGCCTCCTAAAC | 2977 |
| SSR2 | CTTTTACTACCTTGTCTTG | |||
| F3 | 12S | SSF3 | CTGACANAACADGTGCCAGC | 551 |
| SSR3 | CTATGTTACGACWTGCCTCY | |||
| F4 | 12S-CYTB | SSF4 | CAAAGATAAACTTTTACCTCAGG | 1439 |
| SSR4 | GTTATGAAGGATGCTGTTGGG | |||
| F5 | CYTB | SSF5 | GAATATGAACAGGTGTTCTC | 420 |
| SSR5 | TGGMGTATGTTCTNCCTTGG | |||
| F6 | CYTB-NAD4 L | SSF6 | GAGTTACTCCTCTTACAGC | 3881 |
| SSR6 | GTCTCATTGTTAGCTTTGGAG | |||
| F7 | NAD4 L-16S | SSF7 | CCATATTCATTTTATCTCTTCC | 1113 |
| SSR7 | CGTAATCGTATTTGGGAGTTC | |||
| F8 | 16S | SSF8 | GATAGAAAHCAACCTNGCTTAC | 490 |
| SSR8 | GTAGVCTCTGTTCAATGATGAC | |||
| F9 | 16S-ATP6 | SSF9 | GGGTCTTGTCGTCCCTTTAG | 4553 |
| SSR9 | TCAATAGGTGTAAGGTAG |