Surface plasmon resonance spectroscopic analysis of HCMV and EBV NEC protein-peptide interactions.
A, binding of pUL50 to the pUL53 hook peptide. B, binding of BFRF1 to the BFLF2 hook peptide. C, binding of pUL50 to the BFLF2 hook peptide. D, binding of BFRF1 to the pUL53 hook peptide. For each interaction, at least two independent experiments have been performed; one representative set of sensograms is shown. All experiments were performed using the same batches of proteins and peptides.