Table 3.
Crystallographic data collection and refinement statistics
| Structure | EBV BFRF1::BFLF2 | HCMV pUL50::pUL53 |
|---|---|---|
| Data collection | ||
| Beamline | BESSY II 14.2 | BESSY II 14.2 |
| Wavelength (Å) | 0.9182 | 0.9182 |
| Space group | P6122 | P21 |
| Unit cell parameters (Å, °) | a = b = 59.3, c = 265.4, α = β = 90, γ = 120 | a = 37.3, b = 82.6, c = 63.7, α = γ = 90, β = 95.1 |
| Resolution range (Å) | ||
| Spherical | 47.90–1.56 (1.74–1.56)a | 41.29–1.48 (1.52–1.48)a |
| Ellipsoidal | 2.00 Å (b* x c* direction) | NAb |
| 2.00 Å (b* direction) | ||
| 1.50 Å (c* direction) | ||
| Crystal mosaicity (°) | 0.041 | 0.068 |
| Unique reflections | 24,237 | 63,159 |
| Multiplicity | 36.9 | 4.0 |
| Completeness | ||
| Spherical (%) | 59.4 (10.7) | 98.7 (96.3) |
| Ellipsoidal (%) | 92.3 (68.6) | NA |
| Rmeas (%) | 7.4 (233.9) | 9.0 (92.9) |
| Rpim (%) | 1.2 (43.2) | 4.5 (46.1) |
| 〈I/σI〉 | 26.5 (1.9) | 8.9 (1.59) |
| CC1/2 | 1.000 (0.804) | 0.993 (0.592) |
| Wilson B (Å2) | 43.4 (56.1 in direction of b* x c* and b*; 24.0 in direction of c*)c | 33.7 |
| Refinement | ||
| Rwork/Rfree (%) | 21.2/24.2 | 19.2/22.5 |
| Mean B (Å2) | 45.4 | 42.8 |
| No. of TLS groups | 7 | 14 |
| No. of copies in ASU | 1 | 2 |
| No. of atoms (non-H) | ||
| Protein | 1,743 | 3,235 |
| Ligands | 7 | 0 |
| Solvent | 101 | 247 |
| DPI (Å)d | 0.200 | 0.095 |
| RMSD from ideal geometry | ||
| Bond lengths (Å) | 0.007 | 0.005 |
| Bond angles (°) | 0.800 | 0.785 |
| Ramachandran statistics (%) | ||
| Favored | 97.6 | 98.97 |
| Outliers | 0.00 | 0.00 |
| Clashscoree | 1.7 | 4.6 |
| PDB code | 6T3Z | 6T3X |