Table 4.

Computational and experimental alanine scan of the HCMV hook peptide

The numbering and residue type at the individual positions refer to HCMV pUL53.

Position replaced with Ala	ΔΔGa	IC50b ± S.D.
	kcal/mol	μm
WT	0	0.11 ± 0.04
Leu-59	1.42	0.89 ± 0.12
Thr-60	−0.06	0.14 ± 0.02
Leu-61	1.76	0.47 ± 0.01
His-62	0.24	0.13 ± 0.02
Asp-63	−0.65	0.45 ± 0.01
Leu-64	1.22	>10
His-65	1.73	0.42 ± 0.01
Asp-66	−0.34	0.12 ± 0.001
Ile-67	1.54	1.30 ± 0.005
Phe-68	1.62	>10
Arg-69	0.78	0.15 ± 0.01
Glu-70	−0.07	1.11 ± 0.01
His-71	0.64	1.16 ± 0.03
Pro-72	−0.03	0.324 ± 0.02
Glu-73	0.10	0.22 ± 0.002
Leu-74	1.09	1.87 ± 0.07
Glu-75	1.80	>10
Leu-76	0.10	0.11 ± 0.003
Lys-77	0.65	0.37 ± 0.05
Tyr-78	2.38	>10
Leu-79	2.18	>10
Asn-80	0.01	0.12 ± 0.01
Met-81	2.98	1.31 ± 0.13
Met-82	3.13	>10
Lys-83	0.60	0.22 ± 0.001
Met-84	0.98	0.22 ± 0.06
Ile-86	1.69	0.53 ± 0.16
Thr-87	0.01	0.16 ± 0.05

^a Positive ΔΔG values indicate a destabilization of the complex upon replacement of the respective residue by alanine.

^b IC₅₀ (inhibition of pUL50-pUL53 interaction) represent means of at least two experiments.