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. 2020 Jan 28;295(10):3239–3246. doi: 10.1074/jbc.RA119.010509

Figure 1.

Figure 1.

MHC-Ib are an extended family of ligands for CD8αα. Staining of RMA-s–CD8 αα cells with class Ia and MHC-Ib tetramers demonstrates selectivity of class I MHC binding. a, staining of CD8α on RMA-s–CD8αα cells. The open histogram is the unstained control whereas the light-shaded histogram is CD8 staining on RMA-s parental cells. The filled histogram is CD8 staining on RMA-s–CD8αα cells. Results are representative of at least three independent experiments. All histograms have been offset to stack vertically above one another and scaled to maximum count for clarity. b, staining of RMA-s–CD8αα cells with class Ia and MHC-Ib tetramers demonstrates that H2-T22, TL, H2-Q10, and Qa-1b bind CD8αα. The open histograms are unstained controls, the gray histograms are the indicated tetramers on RMA-s cells and the filled histograms are tetramer staining on RMA-s–CD8αα cells. Results are representative of at least three independent experiments. All histograms have been offset to stack vertically above one another and scaled to maximum count for clarity. c, median fluorescent intensity (MFI) of H2-T3/TL, H2-Q10 and Qa-1b staining on RMA-s–CD8αα cells. The MFI was pooled from five independent experiments using equivalent tetramer concentrations and identical laser voltages. *, p = 0.0476 and **, p = 0.0079.