Figure 2.
Qa-1b binds CD8αα with high affinity. a and b, binding of (a) decreasing concentrations of CD8αα (1000, 400, 160, and 64 nm; top to bottom) to neutravidin-immobilized Qa-1b or (b) decreasing concentrations of Qa-1b (5000, 2000, 800, and 320 nm; top to bottom) to CD8αα captured by an antibody to CD8α (53–6.72), which was immobilized by amine-coupling. Results are presented in response units (RU) after subtraction of baseline values. Plots are representative of at least two independent experiments. Dotted vertical lines at 0s indicate injection start. Irregular lines represent raw data and solid lines indicate data fit using a 1:1 Langmuir binding model. c, pooled kinetic values from independent SPR experiments, showing mean ± S.E.