Table 2.
Bio-activities of register-shift insulin analogs
| Analog | % AUCa ± S.D. | Kdb |
|---|---|---|
| n | nm ± S.D. | |
| LeuB24 OrnB29c | 61 ± 13 (9) | 0.13 ± 0.03 |
| LeuB25 OrnB29c | 78 ± 11 (9) | 4.9 ± 2.50 |
| GlyB24 LeuB25 OrnB29c | 62 ± 13 (9) | 0.48 ± 0.10 |
| ChaB24 OrnB29c | 53 ± 14 (12) | 0.07 ± 0.05 |
| ChaB25 OrnB29 d | 80 ± 10 (10) | 11.00 ± 2.4 |
| GlyB24 ChaB25–OrnB29c | 63 ± 12 (15) | 0.12 ± 0.02 |
| TyrB24 OrnB29c | 68 ± 8 (10) | 1.73 ± 0.57 |
| TyrB25 OrnB29d | 45 ± 10 (10) | 0.03 ± 0.01 |
| GlyB24 TyrB25 OrnB29c | 72 ± 12 (10) | 0.95 ± 0.19 |
| SerB25 OrnB29 | 76 ± 5 (9) | 2.98 ± 0.7 |
| SerB26 OrnB29 | 52 ± 12 (10) | 0.04 ± 0.01 |
| GlyB24 SerB26 OrnB29 | 79 ± 8 (10) | 1.16 ± 0.17 |
| OrnB29 | 61 ± 12 (35) | 0.07 ± 0.04 |
a Values were calculated from experiments in STZ-rendered diabetic rats (Fig. 3).
b Kd values were calculated from in vitro receptor-binding assays shown in Fig. 2.
c Data displayed are statistically significantly different from the B25 analog as determined by two-sided Student's t test with a significance level of 0.05.
d Data displayed are statistically significantly different from B24 analog as determined by two-sided Student's t test with a significance level of 0.05.