Table 4.
Analog | Hexamer lifetimes (t½) | Calculated SEC massa |
---|---|---|
min ± S.D. | kDa | |
WT insulin | 8.2 ± 1.3 | 6.8 |
Insulin lispro | 4.6 ± 0.3 | 4.2 |
OrnB29 | 8.2 ± 0.8 | 6.6 |
SerB24 OrnB29 | 2.3 ± 0.2 | 4.9 |
GlyB24 OrnB29 | 1.2 ± 0.2 | 4.9 |
a Proteins were made 0.6 mm in a zinc-free buffer and applied to the SEC column as described under “Experimental procedures”; masses were calculated from Fig. S9.