Figure 1.

Roles of RclR and RclA in macrophage survival. RAW264.7 macrophage cells were infected with Salmonella typhimurium SL1344 (WT) and its isogenic strains rclR and rclA. At 2 h (A) and 24 h (B) post-infection, the macrophage cells were lysed, and the number of intracellular bacteria was determined by plating on LB agar. The results are expressed as the mean ± S.D. of four independent experiments. Asterisks indicate a statistically significant difference between WT and mutant strains (***, p < 0.001).