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. 2020 Jan 31;295(10):3099–3114. doi: 10.1074/jbc.RA119.010589

Figure 5.

Figure 5.

2-DG modulates cytokine production by MDM by inducing UPR. A, levels of XBP1S mRNA in MDM stimulated with M-triDAP or LPS in ordinary medium without or with 2-DG (50 mm). Mean ± S.D., n = 8. ***, p < 0.001 compared with cells treated with M-triDAP alone; #, p < 0.05; ###, p < 0.001 compared with cells treated with LPS alone at the same time point. B, levels of XBP1S mRNA upon M-triDAP or LPS stimulation in ordinary or glucose-free medium. Mean ± S.D., n = 4. C and D, cells were pre-treated with 2-DG (50 mm) or glucose-free medium for 30 min, then cultured with or without M-triDAP for 2 h, after which p38 phosphorylation was assessed (C, a representative experiment, D, densitometry; mean ± S.D. of 5 donors). E–J, MDM were pre-treated for 30 min with 2-DG and d-mannose at indicated concentrations in ordinary medium, stimulated with M-triDAP for indicated time periods, after which levels of XPB1S mRNA (E), phospho-p38 (F), TNF mRNA (G and H) and TNF in supernatant (I and J) were assessed. Three experiments per data point, p values by paired t test. In I and J, results were normalized to cells treated with M-triDAP without 2-DG or mannose.