Skip to main content
. 2020 Jan 27;295(10):3228–3238. doi: 10.1074/jbc.RA119.010604

Figure 2.

Figure 2.

GSDMD SNPs at PTM sites conserve normal pyroptotic function. A, cell death as measured by LDH release in response to expression of full-length (FL) SNP-PTM sites in HEK293T cells. Data represent three independent experiments, each with technical triplicates. **, p < 0.01; n.s., no significance. B, cell death resulting from expression of p30-GSDMD SNP-PTM sites in HEK293T cells. Data represent three independent experiments, each with technical duplicates. ****, p < 0.0001. C, Western blot of the stable reconstitution of SNP-PTM sites in Gsdmd−/− iBMDM cells. D, cell death measured by LDH release of reconstituted iBMDMs. E, PI uptake of SNP-PTM reconstituted iBMDMs in response to LPS with 10 μm compared with the same empty and WT GSDMD controls. F, Western blot of stable reconstitution of SNP-PTM sites in GSDMD−/− THP-1 cells. G, cell death measured by LDH release of reconstituted THP-1 cells. H, PI uptake of SNP-PTM reconstituted THP-1 cells in response to LPS with 10 μm compared with the same empty and WT GSDMD controls. In A, B, D, E, G, and H, data represent three or more independent experiments.