Figure 3.

Genetic diversity in GSDMD leads to phenotypic variation in pyroptotic cell death. A, expression of FL structural SNPs in HEK293T cells. n.s., no significance. B, expression of p30 structural SNPs in HEK293T cells. *, p < 0.05; **, p < 0.01. C, stable reconstitution of SNPs into Gsdmd^−/− iBMDM cells. D, pore formation as measured by PI uptake in reconstituted iBMDMs after priming with LPS and activation with nigericin. All SNPs were from the same experimental sets and compared against the same empty and WT controls. E, cell death as measured by LDH release in reconstituted iBMDM cells primed with LPS and activated with nigericin. ****, p < 0.0001. In A, B, D, and E, data represent three or more independent experiments.