Figure 5.

Changes in polyamine levels and metabolic enzyme activities in Me₂SPM-treated mouse brain, muscle, and heart. Brain tissue from treated mice was assayed for SSAT (A) and ODC (B) activities and intracellular PAs (C). Skeletal (D) and heart muscles (E) were analyzed for native polyamine distributions. The data points in A and B indicate the average of three determinations per mouse, relative to total protein, with horizontal lines indicating the means with S.E. The data in C–E represent duplicate determinations of each tissue (brain and muscle, mock, n = 7; 10 mg/kg, n = 7; 50 mg/kg, n = 6; heart, n = 2 each). For box-and-whisker plots, the horizontal lines indicate medians, + indicates the means, the boxes span the interquartile range, and the whiskers indicate the extremes.